Figure 9. Depletion of Dsh resulted in the radial formation of multiple membrane invaginations and randomized the cell division axis in the 11th cell cycle epidermal cells.
(A) The directional invaginations labeled by PH-GFP in the control MO-injected cells. The bar represents 10 µm (left). Rose diagrams showing the angle of the invagination relative to the embryonic A–P axis, n = 166 invaginations from three embryos (middle), and the angle of cell division relative to the embryonic A–P axis, n = 108 cells from three embryos (right). The results are almost same as the normal embryos in Figure 1 and Figure 2. (B) The omini-directional invaginations labeled by PH-GFP in the Dsh MO-injected cells. The bar represents 10 µm (left). Rose diagrams showing the angle of the invagination relative to the embryonic A-P axis, n = 182 invaginations from three embryos (middle), and the angle of cell division relative to the embryonic A-P axis, n = 109 cells from three embryos (right). In the embryos with radially formed invaginations, the orientation of cell division is randomized. (C) Co-injection of Dsh MO and Dsh mRNA restores directional invaginations. The bar represents 10 µm (left). Rose diagrams showing the angle of the invagination relative to the embryonic A-P axis, n = 213 invaginations from three embryos (middle), and the angle of cell division relative to the embryonic A–P axis, n = 137 cells from three embryos (right). The results are reminiscent of normal and the control MO-injected embryos.