Table 3.
Solid Phase immunoadsorption | Combinatorial Peptide Ligand Libraries | Hydrogel nanoparticles | |
---|---|---|---|
Separation method | Subtraction | Equilibration enrichment | Enrichment/size exclusion |
Protein interaction principles | Antigen-antibody interaction | Physicochemical interaction (including hydrophobic, van der Waals, hydrogen bonding) between complementary hexapeptides and proteins | Hydrophobic and electrostatic interactions between the dye bait and protein |
Affinity molecule | Monoclonal or polyclonal antibody | Hexapeptides or hexapeptides+Alcian blue dye | Organic dyes Acrylic acid Vinylsulfonic acid |
Size exclusion | No | No | Yes |
Thermo-reversible | No | No | Yes |
Low abundance protein enrichment | Possible | Yes | Yes |
Affinity | Moderate (antibody dependent) | Weak-Strong | Strong |
Specificity | High | Moderate | Moderate (dependent on the class of dye used as the affinity bait) |