Table 3.
Effect of supplementation of nitrogen source on cellulase production by Penicillium sp.
| Nitrogen source | Dry weight of mycelial mat (mg/flask) | Protein content (mg/ml) | Cellulase | ||
|---|---|---|---|---|---|
| aFPase (FPU/ml) | bCMCase (U/ml) | cΒ-glucosidase (U/ml) | |||
| NH4Cl | 150 | 1.08 | -nd- | 0.70 | 0.34 |
| (NH4)2SO4 | 112 | 0.97 | -nd- | -nd- | 0.12 |
| KNO3 | 165 | 1.00 | -nd- | 1.20 | 1.20 |
| Peptone | 475 | 2.07 | 1.35 | 8.64 | 3.68 |
| Urea | 405 | 1.38 | 0.15 | 3.36 | 1.74 |
| Yeast extract | 490 | 2.38 | 2.70 | 12.00 | 11.35 |
| Control | 378 | 2.28 | 5.99 | 11.99 | 1.98 |
Values represented in the table are averages of results of two experiments
-nd- not detected
aFilter paperase (FPase) is expressed in terms of filter paper units. One unit is the amount of enzyme in the culture filtrate releasing µmol of reducing sugar from filter paper per min
bCarboxymethyl cellulase (CMCase) is expressed in terms of units. One unit is the amount of enzyme releasing 1 µmol of reducing sugar from carboxymethyl cellulose per min
cOne unit of β-glucosidase activity is defined as the amount of enzyme liberating 1 µmol of p-nitrophenol per min