Table 7.
Source | Dry weight of mycelial mat (mg/flask) | Protein content (mg/ml) | Cellulase | ||
---|---|---|---|---|---|
aFPase (FPU/ml) | bCMCase (U/ml) | cΒ-glucosidase (U/ml) | |||
SDS | 350 | 1.62 | -nd- | 9.88 | 1.66 |
SDO | 410 | 1.23 | -nd- | 7.52 | 0.90 |
TritonX-100 | 514 | 2.01 | 3.08 | 11.99 | 1.99 |
Tween-80 | 492 | 1.74 | -nd- | 10.25 | 1.04 |
Tween-20 | 480 | 1.67 | -nd- | 9.88 | 2.84 |
Control | 5.99 | 11.99 | 1.98 |
Values represented in the table are averages of results of two experiments
SDO sodium deoxycholate
-nd- not detected
aFilter paperase (FPase) is expressed in terms of filter paper units. One unit is the amount of enzyme in the culture filtrate releasing 1 1.1 mol of reducing sugar from filter paper per min
bCarboxymethyl cellulase (CMCase) is expressed in terms of units. One unit is the amount of enzyme releasing 1 µmol of reducing sugar from carboxymethyl cellulose per min
cOne unit of 13-glucosidase activity is defined as the amount of enzyme liberating 1 µmol of p-nitrophenol per min