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. 2016 Jul;99:147–154. doi: 10.1016/j.tube.2016.05.014

Table 1.

Primers and conditions for PCR amplification of genetic element.

No. Name Sequence (5′–3′) Tm (°C) Target Product size
1 rpoA1F GCATTCCAGTCGATTCCATC 57 rpoA 560 bp
2 rpoA1R CCAAGATCGCCTTCTGATGT
3 rpoA2F GGACGTCGAAAGGAAGAAGA 57 rpoA 542 bp
4 rpoA2R GTCTCCACGTCCAGGATCAG
5 rpoBF GTAGTCCACGCCGTAAACGG 65 rpoB 601 bp
6 rpoBR ACGTCCATGTAGTCCACCTCAG
7 rpoCF CGAAAACCTCTACCGCGAAC 64 rpoC 650 bp
8 rpoCR GCGACAGGATGTTGTTGGAG
9 inhAproF ATCACCACCGCCGCTGAAGC 65 inhApro 650 bp
12 inhAproR GTTCGGGTACCCGGGAATG
11 inhA1F CTACATCGACACCGATATGAC 57 inhA 600 bp
12 inhA1R GACCGTCATCCAGTTGTAG
13 inhA2F GCATCAACCCGTTCTTCGAC 57 inhA 550 bp
14 inhA2R TAATGCCATTGATCGGTGATAC
15 ahpCproF ACCACTGCTTTGCCGCCACC 70 ahpCpro 340 bp
16 ahpCproR CCGATGAGAGCGGTGAGCTG
17 katGF CCAGCGGCCCAAGGTATC 65 katG 820 bp
18 katGR GCTGTGGCCGGTCAAGAAGAAGT
19 ndhF ATCACCACCGCCGCTGAAGC 64 ndh 589 bp
20 ndhR GTTCGGGTACCCGGGAATG