Table 5.
Identified mutations from drug resistant MTBC.
| Gene (No. of isolates) | DNA mutation | Isolates with specific SNP | Amino acid change | Other mutations |
|---|---|---|---|---|
| inhApro (151) | -8T/C | 5 (3.3%) | – | 5: katG S315T, 2: ndh V117I |
| −15C/T | 24 (15.9%) | 8: inhApro -102G/A | ||
| −17G/C | 1 (0.7%) | – | ||
| −102G/A | 16 (10.6%) | – | 8: katG S315T, 8: inhApro -15C/T | |
| −47G/C | 1 (0.7%) | katG S315T | ||
| katG (151) | G944C | 107 (70.9%) | S315T | 16: inhA G204D, 8: inhApro -102G/A: 5: inhApro -8T/C, 1: inhApro -47G/C, 2: ndh V117I, 2: ahpCpro -88G/A, 1: ahpCpro -142 G/A |
| inhA (151) | G611A∗ | 16 (10.6%) | G204D∗ | 16: katG S315T |
| T233C | 1 (0.7%) | V78A | ndh: K32R; aphCpro: -54C/T | |
| ahpCpro (151) | −54C/T | 1 (0.7%) | – | ndh: K32R; inhA: V78A |
| −88G/A∗ | 2 (1.3%) | – | 2: katG; S315T | |
| −142G/A∗ | 1 (0.7%) | – | katG: S315T | |
| ndh (151) | G349A | 4 (2.6%) | V117I∗ | inhApro; -8T/C katG; S315T |
| C411T | 1 (0.7%) | G137G | katG; S315T | |
| A95G & G349A | 1 (0.7%) | K32R∗ & V117I∗ | ahpCpro: -54C/T, inhA: V78A | |
| rpoB (50) | A1295C | 1 (2.0%) | Q432P | |
| A1295C & T1472G | 1 (2.0%) | Q432P & I491S† | ||
| C1294A | 1 (2.0%) | Q432K | ||
| A1304T | 11 (22.0%) | D435V | ||
| G1303T | 1 (2.0%) | D435Y | ||
| C1332T | 1 (2.0%) | S441L | ||
| A1334G | 2 (4.0%) | H445R | ||
| CA1333/4TG | 2 (4.0%) | H445C | ||
| C1333G | 2 (4.0%) | H445D | ||
| C1333T | 1 (2.0%) | H445Y | ||
| C1349T | 26 (52.0%) | S450L | 2: rpoC G332R, 3: rpoC V483G | |
| rpoC (50) | G994C | 2 (4.0%) | G332R | rpoB; S450L |
| T1448G | 3 (6.0%) | V483G | rpoB; S450L |
The 50 RIF resistant strains include the 49 RIF mono-resistant and/or MDR MTBSS and MAF strains in addition to the single RIF mono-resistant Mbovis. Similarly, the 151 INH Resistant strains include the 109 INH mono-resistant and 40 MDR MTBSS and MAF in 2 INH mono-resistant M.bovis.
∗
Novel Mutation.
†
Previously described in RIF resistant E. coli.