Figure 3.

Model fit to experimental data. The model is able to fit experimental data from Hui and Vale.15 To mimic the experimental conditions, the model included initial conditions of (a) 500 molecules of LCK/μm², (b) 500 molecules of LCK/μm² + 500 molecules of CSK/μm², (c) 50 molecules of LCK/μm², or (d) 50 molecules of LCK/μm² + 500 molecules of CSK/μm². Each graph shows the experimental data (dots) and median model fit (dark lines) with the 50% and 90% confidence intervals (dark and light shaded regions, respectively). The data shows the total amount of phospho-Y394 (blue) and phospho-Y505 (red) over time based on quantitative western blots. The experimental data is normalized by the western blot band intensity at 90 min, and the simulations are normalized by the concentration of LCK at the end of the 90 min simulation.