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. 2016 Aug 10;36(32):8317–8328. doi: 10.1523/JNEUROSCI.0806-16.2016

Figure 4.

Figure 4.

Proteasomal inhibitors also stabilize mHtt in the processes of cultured astrocytes. A, Fluorescent imaging showing that MG132 (5 μm) or epoxomicin (100 nm) treatment blocks the clearance of mHtt in the processes of cultured cortical astrocytes. B, C, Quantitative results of red fluorescence intensity of Htt–Dendra2 at different time points showing that MG132 and epoxomicin, but not BFA (100 nm), can stabilize the levels of Htt–23Q and Htt–130Q in processes of cultured astrocytes. n = 10 (Con), 10 (BFA), 15 (MG132), and 13 (epoxomicin) cells in B; n = 11 (Con), 10 (BFA), 12 (MG132), and 11 (epoxomicin) in C. [*p < 0.05, **p < 0.01, *** p < 0.001, Con vs MG132; #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001, Con vs epoxomicin, two-way RM-ANOVA, followed by Bonferroni's post hoc test (factor 1, time; factor 2, treatment).] Error bars represent SEM. Scale bars, 10 μm. Con, Control.