Figure 5.

Western blot analysis of Htt–Dendra2 expression. A, B, Western blot analysis of cultured Htt-transfected cortical neurons (A) and astrocytes (B) after treatments with MG132 (5 μm), epoxomicin (100 nm), or BFA (100 nm). MG132 and epoxomicin have stronger effects on inhibiting clearance of Htt–130Q than BFA in neurons and astrocytes. C, D, Ratios of Htt–Dendra2 to actins from three independent experiments are presented. MG132, epoxomicin, or BFA treatment time is 16 h. Con, Control; Epo, epoxomicin. *p < 0.05, **p < 0.01, *** p < 0.001, unpaired two-tailed Student's t test. ns, Not significant. Error bars represent SEM.