Figure 6.

Western blot analysis of immunoprecipitated Htt. A, B, Transfected Htt was immunoprecipitated by anti-Htt antibody (EM48) from cultured cortical neurons (A) and astrocytes (B), and IgG was used as a control. The immunoprecipitates were probed with antibodies to Htt (EM48) or K48 ubiquitination. MG132 at 5 μm was used to inhibit proteasomal activity. C, Ratios of K48 ubiquitinated Htt to the immunoprecipitated Htt from three independent experiments are presented beneath the blots. *p < 0.05, unpaired two-tailed Student's t test. Error bars represent SEM.