Figure 3.

Secreted factors likely do not underlie KO-macrophage-enhanced neurite. Unlike cocultures, in which KO macrophages augment axon growth, transfer of macrophage-conditioned media (CM) from miR-155 KO macrophages does not enhance neurite outgrowth compared with WT macrophage-CM. A, DRG neurons were cultured in the presence of WT/miR-155 KO macrophage-CM for 48 h. B, C, miR-155 KO macrophage-CM (control or inflammatory) did not significantly enhance neurite branching, initiation, or length. Scale bar, 200 μm.