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. 2016 Jul 27;2016:5378567. doi: 10.1155/2016/5378567

Figure 2.

Figure 2

β-catenin knockdown promoted apoptosis and sensitivity to cisplatin but suppressed proliferation in SCC-25 cells. (a) Transfection efficiency of the cells was evaluated by the fluorescence microscopy (A: OX cells, B: OX-NC cells, C: LX cells, and D: LX-NC cells). (b) Gene expression of β-catenin was confirmed by PCR. (c) Protein expression of β-catenin was confirmed by Western blot analysis. (d) β-catenin knockdown caused a significant decrease in cisplatin IC50 of SCC-25 cells. (e) CCK-8 assay showed β-catenin knockdown suppressed proliferation in SCC-25 cells. (f) Flow cytometry analysis showed β-catenin knockdown promoted apoptosis (OX: overexpression, OX-NC: overexpression-negative control, LX: low expression, and LX-NC: low expression-negative control). ∗ P < 0.05; ∗∗ P < 0.01 (magnification: 100х).