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. 2016 Jun 1;6(8):2407–2419. doi: 10.1534/g3.116.030866

Figure 4.

Figure 4

Supersized LD phenotype and peroxisome import defect of class I mutants drop-5 and drop-9. drop-5(ssd72)ts animals grew supersized LDs (arrow) when shifted from the permissive temperature of 15° to the restrictive temperature of 20° for 36 hr (A). Supersized LDs were abolished by acs-22 null mutation (C). Supersized LDs were partially resistant to fasting-induced hydrolysis (E). The non-ts mutant drop-9(ssd213) is similar to drop-5(ssd72)ts in supersized LD phenotype, dependence on ACS-22, and resistance to hydrolysis (B, D, and F). At the permissive temperature of 15°, GFP::DAF-22 and mRFP::PTS1 proteins in drop-5(ssd72)ts are imported into peroxisomes, displaying a granular pattern (G and H); at 20°, GFP::DAF-22 is not imported, displaying a diffuse pattern (I). mRFP::PTS1 is not imported in drop-9(ssd213) (J). For (G–J), > 50 animals were examined under fluorescence stereoscope, and at least five typical animals were imaged on confocal microscope.