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. 2016 Jun 1;6(8):2407–2419. doi: 10.1534/g3.116.030866

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Copyright © 2016 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Supersized LD phenotype of class III mutants drop-1 and drop-8. drop-1(ssd9)^ts and drop-8(ssd89)^ts animals form supersized LDs after being shifted from the permissive temperature of 20° to the restrictive temperature of 30° for 4 hr (A–D). The formation of supersized LDs is accompanied by an obvious disappearance of small LDs (note the difference between A and C, B and D). Supersized LDs are not dependent on ACS-22 (E and F) and are almost completely hydrolyzed by fasting (G and H). To visualize LD fusion, hjSi56[gfp::dgat-2] was crossed into drop-1(ssd2)^ts. L4 animals were mounted on a confocal microscope and subjected to 30° heat treatment. Time-lapse 3-D confocal fluorescence imaging was conducted. As shown in one example, two LDs (white arrow) fused in a short time window of < 30 sec to form a supersized LD > 5 μm in diameter (I). Each image is an extended focus view of a 9 μm Z-stack. n > 20.