Table 1. Isolation origins of the 13 complementation groups and 118 alleles.
Group | ts | Alleles | Mutagen/Background (Haploid Genomes) | |||||
---|---|---|---|---|---|---|---|---|
EMS/WT | ENU/WT | EMS/Is[mddm] | ENU/Is[mddm] | EMS/acs-22 | ENU/acs-22 | |||
(1.18 × 105) | (1.27 × 105) | (0.95 × 105) | (0.32 × 105) | (1.49 × 105) | (1.49 × 105) | |||
maoc-1 II | 5 | 3 | 2 | x | x | x | x | |
dhs-28 X | 15 | 9 | 6 | x | x | x | x | |
daf-22 II | 23 | 18 | 5 | x | x | x | x | |
prx-10 III | 2 | 2 | x | x | ||||
drop-1 II | ts (38/38) | 38 | 5 | 4 | 2 | 2 | 16 | 9 |
drop-2 IV | 22 | 5 | 5 | 12 | x | x | ||
drop-3 IV | ts (5/5) | 5 | 3 | 2 | x | x | ||
drop-4 X | 1 | 1 | ||||||
drop-5 X | ts (2/2) | 2 | 1 | 1 | x | x | ||
drop-6 X | ts (2/2) | 2 | 2 | x | x | |||
drop-7 | 1 | 1 | x | x | ||||
drop-8 III | ts (1/1) | 1 | 1 | |||||
drop-9 II | 1 | 1 | x | x | ||||
Total | 118 | 45 | 25 | 17 | 4 | 16 | 11 |
The 13 complementation groups consist of four known groups (maoc-1, dhs-28, daf-22, and prx-10), which are separated from the nine new drop groups. Six screen series are defined according to the mutagen and background used. WT, N2 Bristol. Is[mddm], ssdIs1, and ssdIs5[maoc-1; dhs-28; daf-22; mrfp::pts1]. acs-22, a deletion allele acs-22(tm3236). Numbers of alleles are listed. ts, temperature-sensitive alleles; x, no mutant of the group expected due to Is[mddm] bypassing or acs-22 suppression; empty cell, no allele isolated.