Table 2. Allele frequencies with two different mutagens and three backgrounds.
| Mutagen | Background | ||||
|---|---|---|---|---|---|
| EMS | ENU | WT | Is[mddm] | acs-22 | |
| maoc-1 II | 2.5 × 10−5a | 1.6 × 10−5a | 2.0 × 10−5 | ||
| dhs-28 X | 7.6 × 10−5a | 4.7 × 10−5a | 6.1 × 10−5 | ||
| daf-22 II | 15.3 × 10−5a | 3.9 × 10−5a | 9.4 × 10−5 | ||
| prx-10 III | 0.9 × 10−5b | 1.6 × 10−5 | |||
| drop-1 II | 6.4 × 10−5c | 4.9 × 10−5c | 3.7 × 10−5 | 3.1 × 10−5 | 8.4 × 10−5 |
| drop-2 IV | 8.0 × 10−5b | 3.1 × 10−5b | 4.1 × 10−5 | 9.4 × 10−5 | |
| drop-3 IV | 1.4 × 10−5b | 1.3 × 10−5b | 1.2 × 10−5 | 1.6 × 10−5 | |
| drop-4 X | 0.3 × 10−5c | 0.3 × 10−5 | |||
| drop-5 X | 0.9 × 10−5b | 0.4 × 10−5 | 0.8 × 10−5 | ||
| drop-6 X | 1.3 × 10−5b | 0.8 × 10−5 | |||
| drop-7 | 0.6 × 10−5b | 0.4 × 10−5 | |||
| drop-8 III | 0.8 × 10−5c | 0.3 × 10−5 | |||
| drop-9 II | 0.5 × 10−5b | 0.4 × 10−5 | |||
| Total | 43.5 × 10−5 | 22.5 × 10−5 | 28.5 × 10−5 | 16.5 × 10−5 | 9.0 × 10−5 |
a
The number of alleles divided by the number of haploid genomes of the WT screen since Is[mddm] transgene bypasses the selection of and acs-22 suppresses these mutants. In a similar EMS/WT screen in Zhang et al. (2010) and Butcher et al. (2009), allele frequency of maoc-1 is 5.6 × 10−5; dhs-28, 11.1 × 10−5; daf-22, 11.1 × 10−5; prx-10, 2.7 × 10−5.
b
The number of alleles divided by the sum of haploid genomes of the WT and Is[mddm] screens since acs-22 suppresses these mutants.
c
The number of alleles divided by the sum of haploid genomes of the WT, Is[mddm], and acs-22 screens since acs-22 does not suppress these mutants.