Figure 2.

TRAP sequencing read coverage of dsx and fru. Average normalized read coverage (Y axis) across the (A) fru and (B) dsx loci from male (blue) and female (red) TRAP samples aligned to the gene structure. For both fru and dsx, the 5′ end of the transcript is on the right. The gray bar above the count data indicates where there are sex-differences in retention of an exon, because of sex hierarchy regulated alternative pre-mRNA splicing. fru has at least 15 different transcripts, and dsx has at least six different transcripts (Attrill et al. 2016).