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. 2016 Jun 3;6(8):2489–2504. doi: 10.1534/g3.116.030346

Figure 9.

Figure 9

Expression levels of FLO8 and STE11 cryptic transcripts are sensitive to reduced Pol II activity mutants. (A) Northern blotting for FLO8-derived RNAs (left) or STE11-derived RNAs (right) in various mutant strains. Northern blotting for SED1 provided a normalization control. spt6 indicates presence of spt6-1004 allele. (B) Quantification of mRNA levels for FLO8-derived transcripts (full-length, top; major cryptic, middle; and minor cryptic, bottom) normalized to SED1. Full-length FLO8 expression was normalized relative to the level in WT. Levels of cryptic transcripts were normalized to full-length and then relative to those in spt6-1004. Error bars represent standard deviation of the mean for at least three independent biological replicates. (C) Quantification of mRNA levels for STE11-derived transcripts (full-length, top; cryptic 1, middle; and cryptic 2, bottom) normalized to SED1. Full-length STE11 expression was normalized relative to the level in WT. Levels of cryptic transcripts were normalized relative to full-length then to those in spt6-1004. Error bars as in (B). WT, wild-type.