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. 2016 Jun 8;6(8):2531–2542. doi: 10.1534/g3.116.031930

Figure 2.

Figure 2

Cse4 is the only kinetochore protein to consistently cause a growth defect with Mad2 association. (A) A set of 88 kinetochore and kinetochore-associated GFP-tagged proteins was retested to confirm that Mad2 association does not lead to a growth defect. Mad2-GBP is compared with Mad2 alone and GBP alone. The dashed line indicates a mean LGR of 0.4 and the control strains are untagged BY4741, File S2. (B) The same set of 88 GFP strains were retested both with wild-type Mad2-GBP and mad2-RQ/AA-GBP, File S2. (C) The same set of 88 GFP strains were retested with closed Mad2-GBP (C-Mad2-GBP) and compared with GBP alone, File S2. (D) The MAD3 gene was deleted from 21 of the GFP strains listed in (A) and (B). Wild-type MAD3 and mad3∆ GFP strains were retested for their sensitivity to Mad2-GBP and the mad2-RQ/AA-GBP control. The growth defects of Ipl1-GBP and Sli15-GFP with Mad2-GBP are suppressed by mad3∆. (E) Mad2-GBP and mad2-RQ/AA-GBP were imaged in cells encoding either Mtw1-YFP and Ask1-GFP, or Mtw1-YFP and Spc110-CFP. In all cases Mad2 colocalizes with Mtw1 (note that GBP does bind to YFP but not to CFP); scale bars are 5 µm.