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. 2016 Jun 8;6(8):2531–2542. doi: 10.1534/g3.116.031930

Figure 3.

Figure 3

Growth effects of Mad1-kinetochore associations. (A) The 88 GFP-tagged kinetochore-related strains were tested for the effect of Mad1-GBP compared to the average of Mad1 alone and GBP alone. The experiment was performed with 16 replicates, and a number of GFP strains show detectable growth defects. The control strains, indicated in red, were untagged BY4741 (File S3). (B) The growth effects of Mad1-GBP are minimal when compared with an inactive mutant, mad1-A736T-GBP. (C) Mad1-GBP and mad1-A736T-GBP were transferred into 22 GFP strains both with and without the MAD3 gene. The only significant Mad1 SPI, with Cep3, is not suppressed in mad3∆ cells. (D) Fluorescence images of Mad1-GBP or mad1-A736T-GBP in Mtw1-YFP strains that also included either Ask1-CFP or Spc110-CFP (note that GBP does bind to YFP but not to CFP). In many cells there is a distinct population of Mad1-GBP and Mtw1-YFP that localizes distal to the kinetochore (red arrowheads); scale bars are 5 µm.