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. 2016 Jun 7;6(8):2655–2663. doi: 10.1534/g3.116.031674

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Copyright © 2016 Shwartz et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Scc4 is required for cohesin loading. (A) Flowchart of the experimental design. (B) Strains YIO002 (scc4-4), YMS1004 (SCC43V5 scc4-4), YMS1006 (scc4L305ins-3V5 scc4-4), YMS1007 (scc4V485ins-3V5 scc4-4), YMS1005 (scc4L490ins-3V5 scc4-4), and YMS1008 (scc4S505ins-3V5 scc4-4) were processed for ChIP analysis. Scc4, V5 tagged proteins were immunoprecipitated. Precipitated chromosome III CARC1 DNA was analyzed by quantitative PCR (n = 3). (C) Analysis of the MAT CAR on chromosome III. (D) Analysis of centromere III. (E) Analysis of centromere IV.