Figure 5. The lncRNA CHRF negatively regulates miR-489 expression.
(A) qRT-PCR analysis of CHRF levels in mouse fibrotic lung tissue on day 7, 14, 28 (n = 8 for each group) with **P < 0.01 vs. the day 28 saline group. (B) qRT-PCR analysis of CHRF levels in RAW 264.7 cells treated with different doses of silica for 12 h with **P < 0.01 vs. the dose 0 group. (C) qRT-PCR analysis of miR-489 levels in RAW264.7 cells transfected with miR-489 mimic or co-transfected with miR-489 mimic and pUC57 plasmids of CHRF with **P < 0.01 vs. the miR-489 mimic group. (D) qRT-PCR analysis of miR-489 levels in RAW264.7 cells transfected with miR-489 mimic or co-transfected with miR-489 mimic and siRNA against CHRF with *P < 0.05 vs. the miR-489 mimic group. All data are expressed as the means ± SD of at least three independent experiments.