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. 2016 Aug 10;6:30552. doi: 10.1038/srep30552

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Copyright © 2016, The Author(s)

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(A) Schematic timeline of procedure to isolate hESC-derived RGCs by MACS. (B) Quantification of THY1.1 positive RGCs post-MACs. (C–F) Characterisation of enriched hESC-derived RGCs using a panel of known RGC markers: (C) NEFM; (D) HUC/D; (E) E’ and E”) individual and merged images of NEFM and βIII TUBULIN; (F) co-staining of BRN3A and NEFM. The magnified images of the highlighted region are shown in F’) as DAPI and F”) as BRN3A. n > 3 for all markers. Scale bars = 100 μm.