Skip to main content
. 2014 Dec 16;3(4):322–339. doi: 10.3390/microarrays3040322

Figure 2.

Figure 2

Chip-specific summary parameters λ and β in dependence of the amount of hybridized RNA. We computed both parameters for the samples of Gene Logic’s dilution data set where two types of RNA (liver and SNB-19) have been hybridized at varying concentrations with 5 replicate samples for each concentration. In Panel (a) λ increases roughly linear with increasing RNA mass between 1 and 10 µg (Pearson correlations of r > 0.7), but saturates at 20 µg; Panel (b) shows how β decreases with increasing RNA mass. An amount of 10 µg aRNA is recommended for the employed HG-U95A platform.