Figure 1. PARP inhibition or PARP1 deficiency rescues lethality of Brca2^ko/ko mESC.

(a) Workflow to test the rescue of Brca2^ko/ko mESC lethality. (b) Representative Southern blot showing rescue of Brca2^ko/ko mESC by olaparib pretreatment in PL2F7 cells. Asterisks indicate the rescued clones. Ratio of the number of rescued clones and total numbers of HAT^r clones analysed are shown in the box on the right corner (same as below). (c) Western blot showing PARP1 level in stable knockdown clones. N, nonsense. (d) Representative Southern blot showing rescue of Brca2^ko/ko mESC by PARP1 knockdown.