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. 2016 Aug 8;7:12389. doi: 10.1038/ncomms12389

Figure 2. β-Catenin promotes SMC proliferation and survival during artery formation.

Figure 2

(a) Immunohistochemistry (IHC) for the endothelial marker CD31. Arrowheads indicate the endothelial layer. Scale bar, 50 μm. (b) IHC for SMC markers, Sm22α (brown; scale bar, 25 μm) and α-SMA (red; scale bar, 50 μm). Arrowheads delimit the vessel wall. Arrows indicate scattered SMCs. (c) Immunostaining of PDAs for the mitotic marker pHH3 and α-SMA at E10.5. Arrowheads indicate pHH3+ SMCs. Scale bar, 20 μm. (d) Quantification of pHH3+ SMCs in the wall of PDAs. **P<0.01; ***P<0.001 comparing genotypes by two-way ANOVA and Sidak's multiple comparison test. n=6. (e) Immunostaining for cleaved Caspase3 and α-SMA at E11.5. Scale bar, 20 μm (PDA); 50 μm (FDA). (f) Quantification of Caspase3+ SMCs in the arterial wall. **P<0.01 comparing genotypes by two-tailed t-test. n=3. In d,f, data represent the mean±s.e.m.