Figure 5. Loss of p53 suppresses the vascular phenotype caused by loss of β-catenin.

(a) Left: immunostaining for Bax, a known p53 target gene, and α-SMA at E11.5. Scale bar, 20 μm (PDA); 50 μm (FDA). Right: quantification of Bax expression in the media of aortas, expressed as percentage of medial area positive for Bax. Data represent the mean ±s.e.m. *P<0.05 by two-tailed t-test. n=4. (b,e) E12.5 embryos of the indicated genotypes. Scale bar, 1 mm. (c) Percentage of E12.5 embryos of the indicated genotypes in three phenotypic categories: normal, early resorption or advanced resorption. **P<0.01; ****P<0.0001; comparisons done by χ²-test. (d) H&E-stained sections of FDAs of the indicated genotypes. Scale bar, 50 μm.