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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Microbiol. 2016 May 24;101(3):457–470. doi: 10.1111/mmi.13403

Figure 3. Expression of FcpA protein in purified periplasmic flagella of WT, fcpA- mutant and complemented L. interrogans strains.

Figure 3

(A) Coomassie-stained SDS-PAGE of purified flagella from motile Fiocruz LV2756 [lane 1], motility-deficient Fiocruz LV2756 [lane 2], Fiocruz LV2756 fcpA-/+ [lane 3], WT Fiocruz L1-130 [lane 4], Fiocruz L1-130 fcpA- [lane 5], and Fiocruz L1-130 fcpA-/+ [lane 6] strains. In Fig. 3A, arrows indicate the position of FcpA and FlaB1 proteins, which were identified by mass spectroscopy. (B) Immunoblotting analysis of purified flagella incubated with a mixture of polyclonal antibodies against FcpA and control antibodies against flagella-associated proteins, FlaA1 and FlaA2. Arrows indicate the positions of these three proteins in Fig. 3B.