Table 3.

Association of Topo–IIα Amplification With Disease and Treatment Variables

Variable	Topo–IIα Amplified	Topo–IIα Deleted	Topo–IIα Normal	P
HER2 IHC 3+, No. of cases	90	50	1.0	< .0001
FISH, ratio	4.5	1.11	1.05	< .0001
S phase, % nuclei	17.5	14	12	.12
ER, % nuclei	55	61	67	.20

NOTE. The values displayed for HER2 IHC, FISH ratio, and S phase are medians and for ER the percentage positive. Differences in the mean ranks or proportions are compared using the Kruskal–Wallis test.

Abbreviations: Topo–IIα, topoisomerase IIα; IHC, immunohistochemistry; FISH, fluorescence in situ hybridization; ER, estrogen receptor.