Fig. 3.

Demethylation agent Zebularine increased CRYAA mRNA expression in HLE B-3 cells detected by Real time qRT-PCR. A. Zebularine increased CRYAA mRNA expression in a dose-dependent pattern. As the concentration of zebularine increased from 10 μM to 200 μM, the CRYAA mRNA level increased to 4.5 fold of untreated controls after zebularine treatment for 24 h, 25.6 fold for 48 h and 42.0 fold for 72 h. B. Zebularine also increased CRYAA mRNA expression in a time-dependent pattern. After 24 to 72 h with zebularine treatment of different concentrations, the CRYAA mRNA expression level increased to 3.4 fold of the control level in 10 μM concentration, 15.6 fold in 20 μM, 29.1 fold in 50 μM, 78.8 fold in 100 μM and 147.3 fold in 200 μM. *P < 0.05. The error bars represent standard deviation of the mean of 3 experiments