Fig. 4.

Mechanical ventilation after PA instillation induced protein expression of cytokines in the lungs of WT mice, but not in JNK1^−/− mice. a The protein expression of proinflammatory cytokines (TNF-α, IL-1β, MIP-2, and IL-6) in the lungs of WT and JNK1^−/− mice after different treatment was examined by Western blotting. n = 3/group. b The levels of proinflammatory cytokines (TNF-α, IL-1β and IL-6) in the lungs of WT and JNK1^−/− mice after saline or 1 × 10⁶ CFU PA instillation and mechanical ventilation treatment. CFU, colony forming unit; PA, Pseudomonas aeruginosa. *P < 0.05, **P < 0.01. n = 4-6/group