FIG 4.

Antigen detection ELISA using newly developed YFV MAbs. (A) Antigen detection using YF vaccine virus 17D-infected culture fluid (YF-17D ICF). The antigen detection ELISA could detect up to 1.0 × 10³ FFU/ml of YF-17D virus. (B) The antigen detection ELISA could detect up to 2 ng of YFV-E protein. The dotted line represents the cutoff value (calculated as twice the mean absorbance value of the negative control). Uninfected Vero cell culture supernatants and PBS were used as negative controls for panels A and B, respectively.