TABLE 1.
Summary of the properties of the established MAbs analyzed by different serological assays
| MAb clone | Immunogen | Isotype | Indirect IgG ELISAa |
IFA resultb |
YFV-E Western blot result (E. coli)c | Domain specificityd | FRNT50e |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| YF-17D | DENV2 | JEV | YF-17D | Baringo 1 | Baringo 2 | DENV2 | JEV | YF-17D | Baringo 2 | |||||
| 5H2 | E protein | IgG2bκ | + | − | − | ++ | ++ | ++ | − | − | ++ | DoIIR1 | <10 | <10 |
| 4A1 | E protein | IgG1κ | + | − | − | + | + | + | − | − | + | ND | <10 | <10 |
| 4C9 | E protein | IgG1κ | + | − | − | ++ | ++ | ++ | − | − | ++ | DoIR1 | <10 | <10 |
| 4H10 | E protein | IgG2bκ | + | − | − | ++ | ++ | ++ | − | − | ++ | DoIR1 | <10 | <10 |
| 3B6 | 17D virus | IgG2bκ | + | − | − | + | + | + | − | − | + | ND | <10 | <10 |
| 5B6 | 17D virus | IgG1κ | + | − | − | + | + | + | − | − | + | DoIIR1 | <10 | <10 |
| 3F4 | 17D virus | IgG2aκ | + | − | − | ++ | ++ | ++ | − | − | + | DoIR1 | <10 | <10 |
| 8H3 | 17D virus | IgG1κ | + | − | − | ++ | ++ | ++ | − | − | + | ND | <10 | <10 |
a
The reactivity of MAbs with selected flaviviruses was determined by indirect IgG ELISA. +, positive; −, negative.
b
IFA fluorescence intensity was scored as follows: −, no fluorescence detectable; +, intermediate reactivity; ++, high fluorescence intensity.
c
The reactivity of MAbs with YFV-E protein was determined by Western blot analysis. −, no detectable signal; +, weak positive signal; ++, strong positive signal.
d
ND, not determined.
e
FRNT50, neutralization titer. The neutralization titer was determined as the reciprocal of the MAb dilution that reduced the number of foci by 50% or more in wells with MAb compared to negative-control wells.