FIG 2.

Flow cytometry results with different target bacteria and MAbs, which are identified in each graph. (A) The hybridoma supernatants were diluted 1:2 (Hflex2a1), 1:50 (Hsoni1, Hsoni5), or 1:250 (all others). Target bacteria are S. flexneri 2a (black solid line), S. flexneri 3a (gray dashed line), and S. sonnei (black dotted line). Gray filled areas show negative controls obtained with the bacteria (targeted by the MAb) in the absence of MAb. (B) Flow cytometric results with S. flexneri 2b target bacteria stained with Hflex2a4 diluted 1:10 (solid line) or 1:250 (dashed line) and with no MAb (gray filled area). All of the experiments were performed more than two times, and one representative example is shown here.