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. 2016 Aug 10;7:249. doi: 10.3389/fphar.2016.00249

FIGURE 5.

FIGURE 5

Inhibition of GRP78 expression enhanced DT-010-induced cell apoptosis in MCF-7 cells. (A) The percentage of apoptotic cells in MCF-7 cells was recorded after 24 h of DT-010 treatment in the presence or absence of ER stress inhibitor 4-PBA. #P < 0.05 vs. DT-010 treated group. (B) MCF-7 cells were treated with Dox and DT-010 for 24 h, the expression of p53, cleaved-PARP, β-actin proteins were measured by western blot. The expression of GRP78 protein was determined after 12 h of Dox and DT-010 treatment. Representative immunoblots for the expression of p53, cleaved-PARP, β-actin and GRP78 proteins. (C) Densitometric quantification of the expression of GRP78 protein. (D) GRP78 knockdown further promotes DT-010-induced cell death in MCF-7 cells. ∗ means significant difference between treatments (P < 0.05).