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. 2016 Aug;55(2):275–287. doi: 10.1165/rcmb.2015-0185OC

Figure 7.

Figure 7.

Increased Akt1 phosphorylation and the CTMP degradation are recapitulated in lambs with increased pulmonary blood flow. Protein extracts (20 μg), prepared from peripheral lung of shunt and control lambs, were subjected to IB analysis on duplicate gels using antibodies specific to pT308Akt1, pS473Akt1, pS617eNOS, and CTMP. Blots were then stripped and reprobed with antibodies raised against β-actin, Akt1, or eNOS. A representative image is shown for each (A–E). Akt1 protein levels did not change in shunt lambs (A). The levels of pT308Akt1 (B), pS473Akt1 (C), and pS617eNOS (D) were significantly increased, whereas CTMP levels (E) were decreased in shunt lambs. However, CTMP mRNA levels were unchanged (F). Protein extracts (1 mg) were also subjected to IP using an antibody specific to Akt1, then analyzed by IB analysis using an antibody raised against CTMP. A representative image is shown (G). The association of Akt1 with CTMP was significantly reduced in shunt lambs (H). Values are means (±SEM); n = 6–10. *P < 0.05 versus control.