Figure 3.

CXCL1 released from injured epithelium is dependent on MMP7. Type II cells were isolated from WT, Mmp7^−/−, and Sdc1^−/− mice and grown, injured, and co-cultured with neutrophils, as described in Figure 2. (A) Cxcl1 (KC) mRNA was quantified by quantitative RT-PCR. RQ was normalized to the WT/intact values. ^#P < 0.01, relative to intact conditions within the same genotype; *P < 0.01, relative to WT within the same condition. (B) CXCL1 (KC) protein levels in media samples were quantified by ELISA. Data are mean ± SE of cells isolated from three mice/genotype. ^#P < 0.01, relative to intact conditions within the same genotype; *P < 0.01 relative to WT within the same condition. The legend is for both panels.