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Experimental protocol. BALB/c mice were sensitized with PBS or ovalbumin (OVA) plus 2 mg of aluminum hydroxide gel (Alum) on Days 0 and 7 (general sensitization) and then received intranasal PBS or OVA from Day 14 to Day 20. For the nasal polyp (NP) group, 6% OVA with Staphylococcus aureus enterotoxin B (SEB; 10 ng) was instilled into the nasal cavity of mice three times per week for 8 weeks after 2 weeks of the OVA-alone challenge.
