Figure 4.
Antibody levels in NLF. Total Ig isotype assay by the Mouse Ig Isotyping Multiplex kit demonstrated that NLF of the NP group contained significantly increased levels of IgA (A) and IgG1 (B) compared with those seen in control. IgG1 level was also significantly higher in the NP group than that in the OVA group. The levels of IgG2a (C), IgG2b (D), and IgG3 (E) were significantly lower in the NP group than those in control. Data presented are means ± SEM. *P < 0.05 versus control; †P < 0.05 versus OVA group.