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. 2015 Nov 23;1:15055. doi: 10.1038/cddiscovery.2015.55

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Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Morphological observation of nano-/micron-sized COM and COD crystals and cytoskeleton. (a) SEM and TEM images of nano-/micron-sized COM and COD crystals, respectively. (b) Confocal laser scanning microscopy observation of the changes of cytoskeleton caused by nano-/micron-sized COM and COD crystals. Cell nuclei (blue) and cytoskeleton (green, as represented by F-actin) were stained with DAPI and FITC-conjugated phalloidin, respectively. Crystal concentration: 200 μg/ml.