Figure 6.

S. aureus induces release of AIF from mitochondria in retinal Müller glia. MIO-M1 cells were left uninfected (control) or challenged with S. aureus RN6390 (multiplicity of infection, 10 : 1) for the indicated time points. Cells were stained with MitoTracker red (Mito Red) followed by immunostaining for AIF and observed under confocal microscope (a). In another experiment, S. aureus-challenged MIO-M1 cells were subjected to subcellular fractionation followed by western blot for AIF (b). Cox 4 and β-actin antibodies were used as protein loading controls for mitochondrial and nuclear enriched fractions, respectively. Results are representative of two independent experiments.