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. 2015 Oct 19;1:15044. doi: 10.1038/cddiscovery.2015.44

Figure 5.

Figure 5

Cell morphology of HCT116 MUT cells resemble that of WT cells following inhibition of PI3K-p110α. (a) Time-dependent inhibition of A66 on PI3K-p110α. HCT116 MUT cells were cultured in 1 μM A66 containing medium for the indicated time points and immunoblotting analysis was used to determine the phosphorylation of Akt. The maximal inhibition effect was at 6 h. (b) Cellular morphology of HCT116 MUT cells in the presence or absence of A66. HCT116 MUT cells were cultured on coverslips and incubated for 6 h in the medium presence or absence of 1 μM A66. Top panel: cell morphology of the living MUT (±A66) and WT HCT116 cells (×20 magnificence). Bottom panel: confocal images of fixed MUT (±A66) and WT HCT116 cells (×63 magnificence). For the confocal images, cells were fixed and stained for F-actin (green) and nuclei (blue). (c) Shape factor of HCT116 MUT cells in the presence or absence of A66. Quantitative analysis of the cell shape of MUT cells, treated with/without A66, was conducted using shape factors. Shape factors were determined by the area, perimeter and circularity of individual cells. Untreated WT cells were used as the control. For each sample, at least 20 individual cells were included in this assay.