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. 2015 Nov 9;1:15020. doi: 10.1038/cddiscovery.2015.20

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Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Endosulfan-induced cell death is specific to testes. (a) TUNEL assay showing DNA fragmentation in testicular cells following ES treatment (3 mg/kg) in male mice. Green colour indicates methyl green counter-stained nuclei and the brown spots are broken DNA stained with diaminobenzidine (DAB). Control sections are devoid of TUNEL-positive cells. (b) TUNEL assay performed on sections obtained at 11th day post-ES treatment showing TUNEL-positive cells. indicating persisting cell death. (c) TUNEL assay showing no stained cells in control and ES-treated mice lungs (1st day). (d) TUNEL assay in brain sections showing absence of positively stained cells (×20).