Figure 2.

Histological staining of the mice retina after NaIO₃ administration. (a–d) Hematoxylin and eosin staining of paraffin-embedded retinal cross sections of 20 mg/kg NaIO₃-treated C57BL/6J mice, at different time points. At 24 h post administration, loss of RPE pigmentation was visible (arrowheads); RPE swelling and breaking of RPE monolayer was observed at 48 and 72 h, respectively (arrowheads). Scale bar is 25 μm. (e–h) Areas indicated by arrowheads at higher magnification. Scale bar is 10 μm. (i–l) Semi-thin sections and methylene blue staining revealed more details of the degenerated but still partially pigmented RPE cells with visible cell vacuolization at 72 h (arrowhead). Scale bar is 10 μm. ONL: photoreceptor outer nuclear layer, IS: inner segments, OS: outer segments. Results shown here are representative from six C57BL/6J mice at each time point.