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. 2015 Oct 26;1:15017. doi: 10.1038/cddiscovery.2015.17

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Copyright © 2015 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of curcumin on mitochondrial bioenergetics and mitochondrial PTP opening. (a) Trace control. Recordings of ΔΨ_m (TPP⁺ electrode measurements; green line) and oxygen consumption (Clark electrode; black line) in purified mouse liver mitochondria. The numbers in black along the trace show nmol O₂/min/mg protein and the ΔΨ_m is reported in mV. TPP⁺ was added to calibrate the electrode. Measurements were performed in the presence of succinate (1 mM) and rotenone (5 μM) together with a saturating amount of ADP (0.5 mM). The uncoupler, mClCCP (10 μM), was added at the end of the trace to uncouple fully respiration. (b) Effect of 25 μM curcumin on ΔΨ_m (TPP⁺ electrode measurements; green line) and oxygen consumption (Clark electrode; black line) in purified mouse liver mitochondria. The experiment was performed in the same conditions as for trace a. (c) Opening of the mitochondrial permeability transition pore in different conditions; comparison between PTP opening with 25 μM calcium, 5 μM ter-butylhydroperoxide or 25 μM curcumin. CsA was used at 5 μM. One representative experiment (n=6) is shown. (d) Opening of the mitochondrial permeability transition pore in response to increasing concentrations of curcumin (from 2.5 to 100 μM).