Table 1. Regulation of the curcumin-induced production of hydrogen peroxide by various antioxidants.

Conditions	DCF fluorescence (a.u.)
	Control	+Curcumin 25 μM
No addition	8±3a	48±5
+NAC (5 mM)	5±4	21±8
+Trolox (1 mM)	6±5	17±6
+Vitamin E (1 mM)	7±4	18±6
+MitoQ10 (500 nM)	1±4	11±7
+SKQ1 (25 nM)	1±3	10±6

Abbreviation: DCF, dichlorofluorescein.

Huh-7 cells where incubated for 24 h with 25 μM curcumin and then stained for 30 min at 37 °C with dichlorofluorescein diacetate. The resultant dichlorofluorescein fluorescence is expressed as the percentage of fluorescent cells that are more fluorescence or less fluorescent than control cells.

^aCorresponds to basal ROS detection (i.e., control cells).