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. 2016 Apr 25;2:16028. doi: 10.1038/cddiscovery.2016.28

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Copyright © 2016 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Tcf7l1 and LCN2 expression in cells in vitro. Control HFKs (transfected with blank vector), E6/E7 gene-transfected HFKs, and SCC-13 cells were cultured in vitro. (a) Immunofluorescence showed the strongest Tcf7l1 (green) and LCN2 (red) staining in SCC-13 cells, followed by E6/E7-transfected and control HFKs in order. (b and c) Flow cytometry confirmed more Tcf7l1 and LCN2 expression in SCC-13 cells and E6/E7-transfected HFKs when compared with control HFKs. MFI, mean fluorescent intensity. (d and e) Western blot revealed the highest levels of Tcf7l1 (d) and LCN2 (e) in SCC-13 cells. Data show mean±S.E.M. of triplicate cultures (n=3). Statistical differences were tested with one-way ANOVA followed by a two-tailed t-test. Representative images are shown. Scale bar=5 μm. *P<0.05; **P<0.01.