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. 2016 Apr 25;2:16028. doi: 10.1038/cddiscovery.2016.28

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Copyright © 2016 Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The effect of Tcf7l1 depletion on LCN2 expression in HFKs. HFKs were stimulated with calcium chloride (1.2 mM, 7 days) after siRNA transfection. (a and b) Intracellular (a) and soluble (b) LCN2 levels were evaluated by western blot and ELISA, respectively. (c) By immunofluorescence, the expression of involucrin and loricrin were detected in HFKs that were transfected with Tcf7l1 or control siRNA. (d and e) The expression of involucrin and loricrin was also detected in HFKs by western blot. Data show mean±S.E.M. of triplicate cultures (n=3). Statistical differences were tested with one-way ANOVA followed by a two-tailed t-test. Representative images are shown. Scale bar=10 μm. *P<0.05; **P<0.01.