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. 2016 Jun 13;2:16039. doi: 10.1038/cddiscovery.2016.39

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Copyright © 2016 Official Journal of the Cell Death Differentiation Association

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative microscopy images of untreated control, WHT-treated and mEHT-treated cells, 3 h post-treatment in Giemsa stained cell samples (a). Representative flow cytometric histogram of the Annexin V-FITC and PI staining 3 h after treatment. The quantitative analysis shows a significant increase of Annexin V positive-cell fraction only in mEHT samples (b). Quantitative results from DNA fragmentation assay. The mEHT-treated cells showed a significant increase in DNA fragmentation percentage, which is one of the hallmarks of apoptotic cell death (c). The results are presented as the mean±S.D. (n=3). **P<0.01 as compared to WHT treatment.