Figure 3.

SH-SY5Y cells express all of the major components required for TRAIL-induced apoptosis. (a) Ethidium bromide-stained agarose gels and (b) western blots demonstrating similar RT-PCR product and protein levels for all of the major components involved in TRAIL-induced apoptosis in NT-SH-SY5Y, pcDNA SH-SY5Y and TrkA SH-SY5Y cells. (c) Representative immunofluorescent micrographs demonstrating close similarity in the expression of functional DR4 and DR5 and DcR1 and DcR2 TRAIL receptors in permeabilized cells and similar levels of cell surface DR4 and DR5 immunoreactivity in nonpermeabilized NT-SH-SY5Y, pcDNA SH-SY5Y and TrkA SH-SY5Y cells (bar=50 μM).